Coding

Part:BBa_K3279004

Designed by: Shujie Liao   Group: iGEM19_CAU_China   (2019-10-14)


beta-carotene 3-hydroxylase (CrtZ)

This part is a beta-carotene 3-hydroxylase which transformsβ-carotene into Zeaxanthin. It is a crucial enzyme in astaxanthin synthesis pathway and always works together with zeaxanthin 4-ketolase (BKT). This sequence is optimized for use in E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 226
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 514
    Illegal PstI site found at 226
    Illegal PstI site found at 436
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 226
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 226
    Illegal PstI site found at 436
    Illegal AgeI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None